Direct Detection ntawm Viral Antibody

Cov txheej txheem no yog kev soj ntsuam los ntawm kev siv cov tshuaj tiv thaiv kab mob tshwj xeeb txhawm rau kuaj cov tshuaj tiv thaiv kab mob hauv cov neeg mob cov ntshav, suav nrog IgM tshuaj tiv thaiv kab mob thiab ntsuas IgG cov tshuaj tiv thaiv.Cov tshuaj tiv thaiv IgM ploj hauv ob peb lub lis piam, thaum cov tshuaj tiv thaiv IgG tseem nyob rau ntau xyoo.Tsim kom muaj kev kuaj mob ntawm tus kab mob kis tau ua tiav los ntawm kev ua kom pom qhov nce hauv cov tshuaj tiv thaiv kab mob rau tus kab mob los yog los ntawm kev ua kom pom cov tshuaj tiv thaiv kab mob ntawm cov chav kawm IgM.Cov txheej txheem siv suav nrog kev kuaj nruab nrab (Nt), qhov ntsuas kev sib txuas ntxiv (CF), kev kuaj hemagglutination inhibition (HI), thiab kev kuaj immunofluorescence (IF), passive hemagglutination, thiab immunodiffusion.

A. Neutralization Assay

Thaum kis kab mob lossis kab lis kev cai ntawm tes, tus kab mob tuaj yeem cuam tshuam los ntawm nws cov tshuaj tiv thaiv tshwj xeeb thiab ua rau tsis muaj kab mob, hom tshuaj tiv thaiv kab mob no txhais tau tias yog cov tshuaj tiv thaiv kab mob nruab nrab.Neutralization assay yog txhawm rau txheeb xyuas cov tshuaj tiv thaiv kab mob hauv cov neeg mob cov ntshav.

B. Ua kom tiav Fixation Assays

Kev ntsuam xyuas ntxiv tuaj yeem siv los saib seb muaj cov tshuaj tiv thaiv tshwj xeeb lossis antigen hauv tus neeg mob cov ntshav.Qhov kev ntsuam xyuas siv cov qe ntshav liab (SRBC), cov tshuaj tiv thaiv SRBC thiab ua kom tiav, nrog rau cov tshuaj tiv thaiv tshwj xeeb (yog tias nrhiav cov tshuaj tiv thaiv kab mob hauv cov ntshav) lossis cov tshuaj tiv thaiv tshwj xeeb (yog tias nrhiav antigen hauv cov ntshav).

C. Hemagglutination Inhibition Assays

Yog tias qhov concentration ntawm tus kab mob hauv ib qho qauv yog siab, thaum cov qauv sib xyaw nrog RBCs, lub lattice ntawm cov kab mob thiab RBCs yuav raug tsim.Qhov tshwm sim no hu ua hemagglutination.Yog tias muaj cov tshuaj tiv thaiv kab mob tiv thaiv hemagglutinins, hemagglutination yuav raug tiv thaiv.Thaum lub sij hawm kuaj hemagglutination inhibition, serial dilutions ntawm cov ntshav yog tov nrog ib tug paub tus nqi ntawm tus kab mob.Tom qab incubation, RBCs ntxiv, thiab cov sib tov yog sab laug rau zaum ob peb teev.Yog tias hemagglutination yog inhibited, ib qho pellet ntawm RBCs tsim nyob rau hauv qab ntawm lub raj.Yog tias hemagglutination tsis yog inhibited, ib zaj duab xis nyias yog tsim.